Editorial Type:
Article Category: Research Article
 | 
Online Publication Date: 01 Jun 2002

Use of Fluorescent-Amplified Fragment Length Polymorphism for Species Identification in the Genus Pulmonaria

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Page Range: 110 – 113
DOI: 10.24266/0738-2898-20.2.110
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Abstract

The species identity of ‘Roy Davidson’, a popular commercial cultivar in the genus Pulmonaria, is uncertain. To determine to which species ‘Roy Davidson’ is related to, we used fifteen primer combinations for Fluorescent-Amplified Fragment Length Polymorphism (F-AFLP) analysis. UPGMA dendrograms generated from AFLP markers obtained from the primer combinations showed that ‘Roy Davidson’ shares more genetic information with P. longifolia than with P. saccharata. Several factors that can affect F-AFLP profiles (DNA isolation from leaf tissue, optimization of F-AFLP procedures, and the use of this method for genotyping pulmonarias) were also evaluated; the results are discussed in this paper.

Keywords: Pulmonaria ; Lungwort; F-AFLP; AFLP; DNA fingerprinting; breeding; Lungworts (Pulmonaria longifolia ‘Bertram Anderson’; P. saccharata ‘White Wings’ and ‘Pierre Pure Pink’; P. longifolia (or saccharata) ‘Roy Davidson’; P. hybrida ‘Majeste’; P. hybrida ‘Victoria Brooch’)
Copyright: Copyright, All Rights Reserved 2002

Contributor Notes

We thank Drs. Chris Catanzaro and Nick Gawel, CARP, Tennessee State University, TN for their helpful review of an earlier draft of the manuscript. This research was partially funded by a Rise Grant, 2001, Tennessee State University.

2Post Doctoral Research Associate.

3Principal Investigator. To whom all correspondence should be directed.

Received: 03 Jan 2002
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