A Shoot-Tip Culture Micropropagation System for Chokecherry
A micropropagation system was developed to clonally propagate juvenile and mature chokecherry plants. Establishment of shoot tips from seedling plants was best on Murashige and Skoog (MS) medium with 5 μM benzyladenine (BA) and 5 μM indolebutyric acid (IBA). Significantly more shoots proliferated on the MS or Driver and Kuniyuki Walnut (DKW) medium than on Woody Plant medium. Rooting of microshoots could be induced on medium with either IBA or naphthaleneacetic acid (NAA). The highest rooting percentage and the greatest number of roots occurred when shoots were placed in a medium with 0.5 or 2.5 μM IBA. More than 20 chokecherry plants shown to have a high level of field tolerance to X-disease were micropropagated for further X-disease resistance testing using the technique described here.Abstract
Contributor Notes
This research was supported in part by USDA-NRICGP 9504248 and Mclntire-Stennis project ND6209. We thank the USDA Plant Materials Center in Bismarck, ND, for making the plant materials available for this research. We also acknowledge Wayne Sargent for helpful suggestions and assistance and Drs. L. Dahleen and A. Lafta for reviewing the manuscript.
2Professor, Department of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu Prov., P. R. China 210095.
3Graduate Student Assistant.
4Associate Professor. To whom reprint requests should be addressed.
5Research Associate, Department of Plant Pathology, North Dakota State University, Fargo, ND 58105.